CD11b B Cells Increase after Stroke and Regulate Microglia

Authors

• Janelle M. Korf, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Pedram Honarpisheh, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Eric C. Mohan, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Anik Banerjee, University of Texas MD Anderson Cancer Center, UTHealth Graduate School of Biomedical Sciences, Houston, TX.
• Maria P. Blasco-Conesa, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Parisa Honarpisheh, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Gary U. Guzman, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Romeesa Khan, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Bhanu P. Ganesh, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Amy L. Hazen, University of Texas McGovern Medical School, Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, Houston, TX.
• Juneyoung Lee, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Aditya Kumar, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Louise D. McCullough, Department of Neurology, University of Texas McGovern Medical School, Houston, TX.
• Anjali Chauhan, Department of Neurology, University of Texas McGovern Medical School, Houston, TX; anjali.chauhan@uth.tmc.edu.

Document Type

Article

Abstract

Recent studies have highlighted the deleterious contributions of B cells to post-stroke recovery and cognitive decline. Different B cell subsets have been proposed on the basis of expression levels of transcription factors (e.g., T-bet) as well as specific surface proteins. CD11b (α-chain of integrin) is expressed by several immune cell types and is involved in regulation of cell motility, phagocytosis, and other essential functions of host immunity. Although B cells express CD11b, the CD11b subset of B cells has not been well characterized, especially in immune dysregulation seen with aging and after stroke. Here, we investigate the role of CD11b B cells in immune responses after stroke in young and aged mice. We evaluated the ability of CD11b B cells to influence pro- and anti-inflammatory phenotypes of young and aged microglia (MG). We hypothesized that CD11b B cells accumulate in the brain and contribute to neuroinflammation in aging and after stroke. We found that CD11b B cells are a heterogeneous subpopulation of B cells predominantly present in naive aged mice. Their frequency increases in the brain after stroke in young and aged mice. Importantly, CD11b B cells regulate MG phenotype and increase MG phagocytosis in both ex vivo and in vivo settings, likely by production of regulatory cytokines (e.g., TNF-α). As both APCs and adaptive immune cells with long-term memory function, B cells are uniquely positioned to regulate acute and chronic phases of the post-stroke immune response, and their influence is subset specific.

Medical Subject Headings

Animals; B-Lymphocytes (metabolism); CD11b Antigen (metabolism); Cell Count; Cytokines (metabolism); Mice; Mice, Inbred C57BL; Microglia (metabolism); Stroke (metabolism)

Publication Date

7-15-2022

Publication Title

Journal of immunology (Baltimore, Md. : 1950)

E-ISSN

1550-6606

Volume

209

Issue

2

First Page

288

Last Page

300

PubMed ID

35732342

Digital Object Identifier (DOI)

10.4049/jimmunol.2100884

Recommended Citation

Korf, Janelle M.; Honarpisheh, Pedram; Mohan, Eric C.; Banerjee, Anik; Blasco-Conesa, Maria P.; Honarpisheh, Parisa; Guzman, Gary U.; Khan, Romeesa; Ganesh, Bhanu P.; Hazen, Amy L.; Lee, Juneyoung; Kumar, Aditya; McCullough, Louise D.; and Chauhan, Anjali, "CD11b B Cells Increase after Stroke and Regulate Microglia" (2022). Neurology. 1664.
https://scholar.barrowneuro.org/neurology/1664