Iptakalim Hydrochloride Protects Cells Against Neurotoxin-Induced Glutamate Transporter Dysfunction In In Vitro And In Vivo Models

Department

neurobiology

Document Type

Article

Abstract

Iptakalim hydrochloride (Ipt), a novel antihypertensive drug, exhibits KATP channel activation. Here, we report that Ipt remarkably protects cells against neurotoxin-induced glutamate transporter dysfunction in in vitro and in vivo models. Chronic exposure of cultured PC12 cells to neurotoxins, such as 6-OHDA, MPP+, or rotenone, decreased overall [ 3H]-glutamate uptake in a concentration-dependent manner. Pre-treatment using 10 μM Ipt significantly protected cells against neurotoxin-induced glutamate uptake diminishment, and this protection was abolished by the KATP channel blocker glibenclamide (20 μM), suggesting that the protective mechanisms may involve the opening of K ATP channels. In 6-OHDA-treated rats (as an in vivo Parkinson's disease model), [3H]-glutamate uptake was significantly lower in synaptosomes isolated from the striatum and cerebral cortex, but not the hippocampus. Pre-conditioning using 10, 50, and 100 μM Ipt significantly restored glutamate uptake impairment and these protections were abolished by blockade of KATP channels. It is concluded that Ipt exhibits substantial protection of cells against neurotoxicity in in vitro and in vivo models. The cellular mechanisms of this protective effect may involve the opening of KATP channels. Collectively, Ipt may serve as a novel and effective drug for PD therapy. © 2005 Elsevier B.V. All rights reserved.

Publication Date

7-5-2005

Publication Title

Brain Research

ISSN

00068993

Volume

1049

Issue

1

First Page

80

Last Page

88

Digital Object Identifier (DOI)

10.1016/j.brainres.2005.04.073

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