Electrophysiological Properties Of Human Hypothalamic Hamartomas

Department

neurobiology

Document Type

Article

Abstract

The hypothalamic hamartoma (HH) is a rare developmental malformation often characterized by gelastic seizures, which are usually refractory to medical therapy. The mechanisms of epileptogenesis operative in this subcortical lesion are unknown. In this study, we used standard patch-clamp electrophysiological techniques combined with histochemical approaches to study individual cells from human HH tissue immediately after surgical resection. More than 90% of dissociated HH cells were small (6-9μm soma) and exhibited immunoreactivity to the neuronal marker NeuN, and to glutamic acid decarboxylase, but not to glial fibrillary acidic protein. Under current-clamp, whole-cell recordings in single dissociated cells or in intact HH slices demonstrated typical neuronal responses to depolarizing and hyperpolarizing current injection. In some cases, HH cells exhibited a \"sag-like\" membrane potential change during membrane liyperpolarization. Interestingly, most HH cells exhibited robust, spontaneous \"pacemaker-like\" action potential firing. Under voltage-clamp, dissociated HH cells exhibited functional tetrodotoxin (TTX)-sensitive Na+ and tetraethylammonium-sensitive K+ currents. Both GABA and glutamate evoked whole-cell currents, with GABA exhibiting a peak current amplitude 10-fold greater than glutamate. These findings suggest that human HH tissues, associated with gelastic seizures, contained predominantly small GABAergic inhibitory neurons that exhibited intrinsic \"pacemaker-like\" behavior. © 2005 American Neurological Association.

Publication Date

9-1-2005

Publication Title

Annals of Neurology

ISSN

03645134

Volume

58

Issue

3

First Page

371

Last Page

382

Digital Object Identifier (DOI)

10.1002/ana.20580

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