Mitochondrial fission inhibition protects against hypertension induced by angiotensin II.

Document Type

Article

Abstract

Mitochondrial dysfunction has been implicated in various types of cardiovascular disease including hypertension. Mitochondrial fission fusion balance is critical to mitochondrial quality control, whereas enhanced fission has been reported in several models of cardiovascular disease. However, limited information is available regarding the contribution of mitochondrial fission in hypertension. Here, we have tested the hypothesis that inhibition of mitochondrial fission attenuates the development of hypertension and associated vascular remodeling. In C57BL6 mice infused with angiotensin II for 2 weeks, co-treatment of mitochondrial fission inhibitor, mdivi1, significantly inhibited angiotensin II-induced development of hypertension assessed by radiotelemetry. Histological assessment of hearts and aortas showed that mdivi1 inhibited vessel fibrosis and hypertrophy induced by angiotensin II. This was associated with attenuation of angiotensin II-induced decline in mitochondrial aspect ratio seen in both the endothelial and medial layers of aortas. Mdivi1 also mitigated angiotensin II-induced cardiac hypertrophy assessed by heart weight-to-body weight ratio as well as by echocardiography. In ex vivo experiments, mdivi1 inhibited vasoconstriction and abolished the enhanced vascular reactivity by angiotensin II in small mesenteric arteries. Proteomic analysis on endothelial cell culture media with angiotensin II and/or mdivi1 treatment revealed that mdivi1 inhibited endothelial cell hypersecretory phenotype induced by angiotensin II. In addition, mdivi1 attenuated angiotensin II-induced protein induction of periostin, a myofibroblast marker in cultured vascular fibroblasts. In conclusion, these data suggest that mdivi1 prevented angiotensin II-induced hypertension and cardiovascular remodeling via multicellular mechanisms in the vasculature.

Keywords

Animals, Angiotensin II, Hypertension, Mitochondrial Dynamics, Mice, Inbred C57BL, Mice, Male, Quinazolinones, Vascular Remodeling, Blood Pressure

Medical Subject Headings

Animals; Angiotensin II; Hypertension; Mitochondrial Dynamics; Mice, Inbred C57BL; Mice; Male; Quinazolinones; Vascular Remodeling; Blood Pressure

Publication Date

5-1-2024

Publication Title

Hypertension research : official journal of the Japanese Society of Hypertension

ISSN

1348-4214

Volume

47

Issue

5

First Page

1338

Last Page

1349

PubMed ID

38383894

Digital Object Identifier (DOI)

10.1038/s41440-024-01610-0

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