Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke

Document Type

Article

Abstract

OBJECTIVES: Infarct volume correlation using magnetic resonance imaging (MRI) and pathology specimens enables exact tissue localization of cerebral injury following experimental stroke. We describe a protocol that enables co-registration of radiographic signal change and histologic ischemia in a non-human primate model of stroke. METHODS: One male baboon underwent left middle cerebral artery territory occlusion/reperfusion. MRI [5 mm axial T2 weighted (T2W) slices] was carried out 9 days post-ischemia after which the animal was killed. Immediately post-mortem, the whole brain was perfused and fixed in paraformaldehyde and sliced into 5 mm axial sections that corresponded to those demonstrated on MRI. Slices (40 microm) were obtained from each section and were then stained using Luxol hematoxylin and eosin. RESULTS: The relative area of hyperintensity demonstrated on T2W MRI approximates, in size and location, the region of infarct on gross pathology. This was confirmed microscopically. DISCUSSION: With the use of advanced imaging modalities, this co-registration technique affords the capacity to differentiate ischemic core, penumbra, and uninjured cortex following experimental stroke. Such a precise delineation enables immunohistochemical analysis of a wide variety of substrates in each of the aforementioned regions.

Medical Subject Headings

Animals; Brain Infarction (diagnostic imaging, pathology); Disease Models, Animal; Magnetic Resonance Imaging (methods); Male; Papio; Radiography; Stroke (complications, diagnostic imaging, pathology)

Publication Date

9-1-2005

Publication Title

Neurological research

ISSN

0161-6412

Volume

27

Issue

6

First Page

634

Last Page

7

PubMed ID

16157015

Digital Object Identifier (DOI)

10.1179/016164105X17305

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