Tau in situ hybridization in normal and alzheimer brain: Localization in the somatodendritic compartment

Document Type

Article

Abstract

Tau messenger RNA in situ hybridization in human postmortem brain revealed that neurons are the predominant cell type labeled. The probe used includes the nucleotide sequence coding for the amino acids recognized by the well‐characterized tau monoclonal antibodies 5E2 and tau 1. The distribution of the tau RNA is abundant throughout the neuronal somata and into the proximal parts of dendrites of pyramidal cells in the cerebral cortex and the hippocamps. The distal extent to which silver grains cold be visualized in the pyramidal cell dendrite was comparable to that seen with a probe to ribosomal RNA. In contrast to the tau probe the ribosomal probe also labeled glial cells. Sections hybridized with the tau probe and then double‐labeled with thioflavine S revealed that neurons containing neurofibrillary tangles tangles continue to synthesize tau protein. Copyright © 1989 American Neurological Association

Publication Date

1-1-1989

Publication Title

Annals of Neurology

ISSN

03645134

E-ISSN

15318249

Volume

26

Issue

3

First Page

352

Last Page

361

PubMed ID

2508535

Digital Object Identifier (DOI)

10.1002/ana.410260308

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