Title

Demonstrating immune-related antigens in Alzheimer's disease brain tissue

Document Type

Article

Abstract

Immune system associated markers have recently been reported in Alzheimer's disease (AD) brain. However, previous studies have not consistently revealed these antigens in the central nervous system. Immunohistochemistry of HLA-DR and other immune antigens (e.g., CD4+, IL-2R) in AD cortical tissue was compared using paraformaldehyde-fixed versus snap-frozen unfixed tissue. In addition, a free-floating immunohistochemistry method was contrasted with an on slide procedure. Even with an HLA-DR antibody known to work well on fixed tissue, unfixed tissue gave superior results. Free-floating immunohistochemistry also increased sensitivity. Where morphologic considerations are paramount, brief fixation with free-floating methods provides a reasonable alternative. © 1990.

Keywords

Brain, Fixation, Formaldehyde, HLA-DR, Neuroimmunology

Publication Date

1-1-1990

Publication Title

Neurobiology of Aging

ISSN

01974580

Volume

11

Issue

4

First Page

477

Last Page

479

PubMed ID

2381508

Digital Object Identifier (DOI)

10.1016/0197-4580(90)90016-S

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