Demonstrating immune-related antigens in Alzheimer's disease brain tissue
Document Type
Article
Abstract
Immune system associated markers have recently been reported in Alzheimer's disease (AD) brain. However, previous studies have not consistently revealed these antigens in the central nervous system. Immunohistochemistry of HLA-DR and other immune antigens (e.g., CD4+, IL-2R) in AD cortical tissue was compared using paraformaldehyde-fixed versus snap-frozen unfixed tissue. In addition, a free-floating immunohistochemistry method was contrasted with an on slide procedure. Even with an HLA-DR antibody known to work well on fixed tissue, unfixed tissue gave superior results. Free-floating immunohistochemistry also increased sensitivity. Where morphologic considerations are paramount, brief fixation with free-floating methods provides a reasonable alternative. © 1990.
Keywords
Brain, Fixation, Formaldehyde, HLA-DR, Neuroimmunology
Publication Date
1-1-1990
Publication Title
Neurobiology of Aging
ISSN
01974580
Volume
11
Issue
4
First Page
477
Last Page
479
PubMed ID
2381508
Digital Object Identifier (DOI)
10.1016/0197-4580(90)90016-S
Recommended Citation
Rogers, Joseph and Mufson, Elliott J., "Demonstrating immune-related antigens in Alzheimer's disease brain tissue" (1990). Translational Neuroscience. 1776.
https://scholar.barrowneuro.org/neurobiology/1776