Axonal control of the adult neural stem cell niche

Authors

Cheuk Ka Tong, Department of Neurological Surgery and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA; Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
Jiadong Chen, Department of Neurological Surgery and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA.
Arantxa Cebrián-Silla, Laboratory of Comparative Neurobiology, Instituto Cavanilles, Universidad de Valencia, CIBERNED, Valencia 46980, Spain.
Zaman Mirzadeh, Department of Neurosurgery, Barrow Neurological Institute, Phoenix, AZ 85013, USA.
Kirsten Obernier, Department of Neurological Surgery and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA.
Cristina D. Guinto, Department of Neurological Surgery and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA.
Laurence H. Tecott, Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Psychiatry, University of California, San Francisco, San Francisco, CA 94158, USA.
Jose Manuel García-Verdugo, Laboratory of Comparative Neurobiology, Instituto Cavanilles, Universidad de Valencia, CIBERNED, Valencia 46980, Spain; Unidad Mixta de Esclerosis Múltiple y Neurorregeneración, IIS Hospital La Fe, Valencia 46013, Spain.
Arnold Kriegstein, Department of Neurological Surgery and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA; Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA.
Arturo Alvarez-Buylla, Department of Neurological Surgery and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA; Neuroscience Graduate Program, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: abuylla@stemcell.ucsf.edu.

Document Type

Article

Abstract

The ventricular-subventricular zone (V-SVZ) is an extensive germinal niche containing neural stem cells (NSCs) in the walls of the lateral ventricles of the adult brain. How the adult brain's neural activity influences the behavior of adult NSCs remains largely unknown. We show that serotonergic (5HT) axons originating from a small group of neurons in the raphe form an extensive plexus on most of the ventricular walls. Electron microscopy revealed intimate contacts between 5HT axons and NSCs (B1) or ependymal cells (E1) and these cells were labeled by a transsynaptic viral tracer injected into the raphe. B1 cells express the 5HT receptors 2C and 5A. Electrophysiology showed that activation of these receptors in B1 cells induced small inward currents. Intraventricular infusion of 5HT2C agonist or antagonist increased or decreased V-SVZ proliferation, respectively. These results indicate that supraependymal 5HT axons directly interact with NSCs to regulate neurogenesis via 5HT2C.

Medical Subject Headings

Animals; Axons (physiology); Blotting, Western; Brain (cytology, physiology); Cell Differentiation; Cell Proliferation; Electrophysiology; Immunoenzyme Techniques; Mice; Microscopy, Electron; Neural Stem Cells (cytology, metabolism); Neurogenesis; Neurons (cytology, physiology); RNA, Messenger (genetics); Raphe Nuclei (cytology, physiology); Real-Time Polymerase Chain Reaction; Receptor, Serotonin, 5-HT2C (chemistry, metabolism); Reverse Transcriptase Polymerase Chain Reaction; Serotonin Receptor Agonists (pharmacology); Stem Cell Niche

Publication Date

4-3-2014

Publication Title

Cell stem cell

E-ISSN

1875-9777

Volume

14

Issue

4

First Page

500

Last Page

11

PubMed ID

24561083

Digital Object Identifier (DOI)

10.1016/j.stem.2014.01.014

Recommended Citation

Tong, Cheuk Ka; Chen, Jiadong; Cebrián-Silla, Arantxa; Mirzadeh, Zaman; Obernier, Kirsten; Guinto, Cristina D.; Tecott, Laurence H.; García-Verdugo, Jose Manuel; Kriegstein, Arnold; and Alvarez-Buylla, Arturo, "Axonal control of the adult neural stem cell niche" (2014). Translational Neuroscience. 1222.
https://scholar.barrowneuro.org/neurobiology/1222