Abnormal balance in the angiopoietin-tie2 system in human brain arteriovenous malformations

Document Type

Article

Abstract

Brain arteriovenous malformations (BAVMs) are congenital vascular lesions that often present as cerebral hemorrhage in young adults. The variable nature of the clinical course, especially with respect to spontaneous hemorrhage, recurrence, growth, and regression, suggests that BAVMs are lesions with active angiogenesis and vascular remodeling. We examined mRNA and protein expression of angiopoietin 1 (Ang1) and Ang2 by semiquantitative reverse transcriptase-polymerase chain reaction, in situ hybridization, and Western blot in BAVMs and control brains obtained from temporal lobectomy for medically intractable seizures. Although Ang1 mRNA levels were similar in BAVMs and controls, Ang1 protein levels were 30% lower in BAVMs than in controls. Ang2 mRNA levels were 40% higher and Ang2 protein levels were 8-fold higher in BAVMs than in controls. In situ hybridization showed that the Ang2 mRNA was localized to the perivascular area in BAVMs. This abnormal balance in the Ang-Tie2 system may, in part, explain the aberrant vascular phenotype in BAVMs.

Medical Subject Headings

Adolescent; Adult; Angiopoietin-1; Angiopoietin-2; Blotting, Northern; Blotting, Western; Brain (metabolism); Female; Humans; In Situ Hybridization; Intracranial Arteriovenous Malformations (genetics, metabolism); Male; Membrane Glycoproteins (genetics, metabolism); Middle Aged; Proteins (genetics, metabolism); RNA, Messenger (genetics, metabolism); Receptor Protein-Tyrosine Kinases (genetics, metabolism); Receptor, TIE-2

Publication Date

7-21-2001

Publication Title

Circulation research

E-ISSN

1524-4571

Volume

89

Issue

2

First Page

111

Last Page

3

PubMed ID

11463715

Digital Object Identifier (DOI)

10.1161/hh1401.094281

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