Isolation and characterization of Y chromosome DNA probes.

Document Type

Article

Abstract

A sorted, cloned Y chromosome phage library was screened for unique Y chromosome sequences. Of the thousands of plaques screened, 13 did not hybridize to radiolabeled 46,XX total chromosomal DNA. Three plaques were characterized further. Clone Y1 hybridized to multiple restriction enzyme fragments in both male and female DNA with more intense bands in male DNA. Clone Y2, also found in female and male DNA, is probably located in the pseudosutosomal region because extra copies of either the X or Y chromosomes increased Y2 restriction enzyme fragment intensity in total cellular DNA. Clone Y5 was male specific in three of four restriction enzyme digests although in the fourth a light hybridizing band was observed in both male and female DNA. Clone Y5 was sublocalized to band Yq 11.22 by hybridization to a panel of cellular DNA from patients with Y chromosome rearrangements. Clone Y5 can be used to test for retention of the proximally long arm Y suggested to cause gonadal cancer in carrier females. The long series of GA repeats in Y5, anticipated to be polymorphic, may provide a sensitive means to follow Y chromosome variation in human populations.

Medical Subject Headings

Base Sequence; Blotting, Southern; Chromosome Banding; DNA; DNA Probes; Female; Gene Library; Humans; Karyotyping; Lymphocytes; Male; Molecular Sequence Data; Plasmids; Sex Chromosome Aberrations; Y Chromosome

Publication Date

11-30-1992

Publication Title

Biochemical and biophysical research communications

ISSN

0006-291X

Volume

189

Issue

1

First Page

581

Last Page

589

PubMed ID

1449506

Digital Object Identifier (DOI)

10.1016/0006-291x(92)91597-j

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