A denaturing gradient gel electrophoresis assay for sensitive detection of p53 mutations

Document Type

Article

Abstract

p53 is a tumor suppressor gene located on 17p, a region of the human genome frequently deleted in tumors. Mutation of the p53 gene is an important step leading to development of many forms of human cancer. To simplify the analysis of tumors for p53 point mutations, we describe a GC-clamped denaturing gradient gel assay for detecting single-base substitutions within highly conserved regions of the p53 gene. This assay allows for efficient screening of tumors for single-base substitutions within the p53 gene and can be used to facilitate sequence analysis of p53 point mutations.

Medical Subject Headings

Base Sequence; Electrophoresis, Gel, Pulsed-Field (methods); Genes, p53 (genetics); Humans; Molecular Sequence Data; Point Mutation (genetics)

Publication Date

3-1-1993

Publication Title

Human genetics

ISSN

0340-6717

Volume

91

Issue

1

First Page

25

Last Page

30

PubMed ID

8454284

Digital Object Identifier (DOI)

10.1007/BF00230217

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