Handheld confocal laser endomicroscopic imaging utilizing tumor-specific fluorescent labeling to identify experimental glioma cells

Authors

Nikolay L. Martirosyan, Department of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center Phoenix, Arizona, USA.Follow
Joseph Georges, School of Life Sciences, Arizona State University, Tempe, Arizona, USA.
M Yashar Kalani, Department of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center Phoenix, Arizona, USA.Follow
Peter Nakaji, Department of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center Phoenix, Arizona, USA.
Robert F. Spetzler, Department of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center Phoenix, Arizona, USA.
Burt G. Feuerstein, School of Life Sciences, Arizona State University, Tempe, Arizona, USA; College of Medicine, University of Arizona, Phoenix, Arizona, USA.
Mark C. Preul, Department of Neurosurgery, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center Phoenix, Arizona, USA.

Document Type

Article

Abstract

BACKGROUND: We have reported that handheld confocal laser endomicroscopy (CLE) can be used with various nonspecific fluorescent dyes to improve the microscopic identification of brain tumor and its boundaries. Here, we show that CLE can be used experimentally with tumor-specific fluorescent labeling to define glioma margins . METHODS: Thirteen rats underwent craniectomy and imaging 21 days after implantation with green fluorescent protein (GFP)-labeled U251 ( = 7) cells or epidermal growth factor receptor (EGFR) overexpressing F98 cells ( = 6). Fluorescein isothiocyanate (FITC) conjugated EGFR fluorescent antibody (FITC-EGFR) was applied for contrast in F98 tumors. Confocal images of normal brain, obvious tumor, and peritumoral zones were collected using the CLE system. Bench-top confocal microscopy and hematoxylin and eosin-stained sections were correlated with CLE images. RESULTS: GFP and FITC-EGFR fluorescence of glioma cells were detected by visible-wavelength fluorescence CLE. CLE of GFP-labeled tumors revealed bright individual satellite tumor cells within peritumoral tissue, a definitive tumor border, and subcellular structures. Imaging with FITC-EGFR labeling provided weaker contrast in F98-EGFR tumors but was able to delineate tumor cells. Imaging with both methods in various tumor regions correlated with standard confocal imaging and clinical histology. CONCLUSIONS: These data suggest that CLE of selectively tagged neoplasms could allow specific interactive identification of tumoral areas. Imaging of GFP and FITC-EGFR provides real-time histologic information precisely related to the site of microscopic imaging of tumor.

Publication Date

1-1-2016

Publication Title

Surgical neurology international

ISSN

2229-5097

Volume

7

Issue

Suppl 40

First Page

S995

Last Page

S1003

PubMed ID

28144472

Digital Object Identifier (DOI)

10.4103/2152-7806.195577

Recommended Citation

Martirosyan, Nikolay L.; Georges, Joseph; Kalani, M Yashar; Nakaji, Peter; Spetzler, Robert F.; Feuerstein, Burt G.; and Preul, Mark C., "Handheld confocal laser endomicroscopic imaging utilizing tumor-specific fluorescent labeling to identify experimental glioma cells" (2016). Neurosurgery. 1688.
https://scholar.barrowneuro.org/neurosurgery/1688