Dysregulation of intestinal crypt cell proliferation and villus cell migration in mice lacking Krüppel-like factor 9

Document Type

Article

Abstract

Krüppel-like factor 9 (Klf9), a zinc-finger transcription factor, is implicated in the control of cell proliferation, cell differentiation, and cell fate. Using Klf9-null mutant mice, we have investigated the involvement of Klf9 in intestine crypt-villus cell renewal and lineage determination. We report the predominant expression of Klf9 gene in small and large intestine smooth muscle (muscularis externa). Jejunums null for Klf9 have shorter villi, reduced crypt stem/transit cell proliferation, and altered lineage determination as indicated by decreased and increased numbers of goblet and Paneth cells, respectively. A stimulatory role for Klf9 in villus cell migration was demonstrated by bromodeoxyuridine labeling. Results suggest that Klf9 controls the elaboration, from intestine smooth muscle, of molecular mediator(s) of crypt cell proliferation and lineage determination and of villus cell migration. Copyright © 2007 the American Physiological Society.

Keywords

Colon, Igfbp4, Krüppel-like, Ptk6, Smooth muscle, Stem cells

Publication Date

6-1-2007

Publication Title

American Journal of Physiology - Gastrointestinal and Liver Physiology

ISSN

01931857

E-ISSN

15221547

Volume

292

Issue

6

PubMed ID

17379758

Digital Object Identifier (DOI)

10.1152/ajpgi.00013.2007

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