Title

The septin CDCrel-1 binds syntaxin and inhibits exocytosis

Document Type

Article

Abstract

Septins are GTPases required for the completion of cytokinesis in diverse organisms, yet their roles in cytokinesis or other cellular processes remain unknown. Here we describe studies of a newly identified septin, CDCrel-1, which is predominantly expressed in the nervous system. This protein was associated with membrane fractions, and a significant fraction of the protein copurified and coprecipitated with synaptic vesicles. In detergent extracts, CDCrel-1 and another septin, Nedd5, immunoprecipitated with the SNARE protein syntaxin by directly binding to syntaxin via the SNARE interaction domain. Transfection of HIT-T15 cells with wild-type CDCrel-1 inhibited secretion, whereas GTPase dominant-negative mutants enhanced secretion. These data suggest that septins may regulate vesicle dynamics through interactions with syntaxin.

Publication Date

5-1-1999

Publication Title

Nature Neuroscience

ISSN

10976256

Volume

2

Issue

5

First Page

434

Last Page

439

PubMed ID

10321247

Digital Object Identifier (DOI)

10.1038/8100

This document is currently not available here.

Share

COinS