Comparative proteomic profiling of cerebrospinal fluid between living and post mortem ALS and control subjects
Document Type
Article
Abstract
Neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), lack definitive diagnostic tests or biomarkers of disease progression. Most studies that investigate protein abnormalities in ALS have used biofluids such as blood or cerebrospinal fluid (CSF), while some have used post mortem tissue or CSF samples. Since ALS disease progression and post mortem effects probably induce significant alterations to protein modifications or proteolysis, we directly examined the CSF proteome from ALS subjects at various lengths of time from symptom onset and at autopsy by mass spectrometry based proteomics. CSF was also obtained from both healthy age-matched control subjects and at autopsy from healthy and Alzheimer's disease (AD) controls. We identified significant differences in the CSF proteome between living and post mortem ALS subjects, as well as living and post mortem control subjects. We also noted differences in the CSF proteome of ALS subjects that have exhibited symptoms for varying lengths of time and between ALS and AD subjects at end-stage of disease. This is the first study describing differences in the CSF proteome from post mortem and living ALS subjects using a mass spectrometric approach. These differences highlight the importance of utilizing CSF from living ALS subjects near the time of symptom onset for the identification of early protein biomarkers, although some protein alterations that occur early in the disease process are maintained throughout the course of disease and in post mortem samples.
Keywords
Biomarkers, Cerebrospinal fluid, Mass spectrometry, Proteomics
Publication Date
12-4-2007
Publication Title
Amyotrophic Lateral Sclerosis
ISSN
17482968
E-ISSN
1471180X
Volume
8
Issue
6
First Page
373
Last Page
379
PubMed ID
17852009
Digital Object Identifier (DOI)
10.1080/17482960701549681
Recommended Citation
Ranganathan, Srikanth; Nicholl, Georgina C.B.; Henry, Sarah; Lutka, Fran; Sathanoori, Ramasri; Lacomis, David; and Bowser, Robert, "Comparative proteomic profiling of cerebrospinal fluid between living and post mortem ALS and control subjects" (2007). Translational Neuroscience. 584.
https://scholar.barrowneuro.org/neurobiology/584