Repeatability and reliability of cerebrovascular reactivity in young adults using multi-echo, multi-contrast MRI

Document Type

Article

Abstract

Cerebrovascular reactivity (CVR) shows promise as a biomarker of vascular integrity and may benefit from a repeatable, reliable, and microvasculature-sensitive acquisition. A combined spin- and gradient-echo (SAGE) functional MRI (fMRI) acquisition may improve repeatability and reliability compared to single spin- (SE) and gradient-echo (GRE) fMRI and provide a microvascular-weighted analysis. The most repeatable and reliable MRI acquisition CVR maps were compared across three CVR paradigms: a breath-hold task, a breath modulation task, and a resting state acquisition. SAGE-fMRI data was acquired in fifteen young adults at two timepoints. Mean gray matter (GM) within-subject coefficient of variation (wCV) and intraclass correlation coefficient (ICC) were compared within the quantitative and weighted SAGE-fMRI CVR maps and single GRE- and SE-fMRI CVR. Total and microvascular MRI inputs with lowest wCV and highest ICC were used to compare three CVR paradigms. Total and microvascular weighted SAGE-fMRI CVR had the lowest wCV and highest ICC across paradigms. The breath-hold paradigm produced significantly higher GM CVR estimates. SAGE repeatably and reliably measures CVR and offers a simultaneous, complementary analysis on total and microvascular scales. The breath-hold paradigm showed significantly higher CVR estimates, but less compliance-dependent protocols may be ideal for applications in patient populations.

Keywords

Cerebrovascular reactivity (CVR), functional MRI (fMRI), multi-echo fMRI, spin- and gradient-echo (SAGE), spin-echo fMRI

Medical Subject Headings

Humans; Male; Magnetic Resonance Imaging (methods); Cerebrovascular Circulation (physiology); Female; Young Adult; Adult; Reproducibility of Results; Gray Matter (diagnostic imaging, blood supply); Brain (blood supply, diagnostic imaging); Breath Holding

Publication Date

10-1-2025

Publication Title

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

E-ISSN

1559-7016

Volume

45

Issue

10

First Page

2030

Last Page

2046

PubMed ID

40415374

Digital Object Identifier (DOI)

10.1177/0271678X251345292

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