The CSF in neurosarcoidosis contains consistent clonal expansion of CD8 T cells, but not CD4 T cells
Document Type
Article
Abstract
The tissue-specific drivers of neurosarcoidosis remain poorly defined. To identify cerebrospinal fluid (CSF) specific, antigen-driven T and B cell responses, we performed single-cell RNA sequencing of CSF and blood cells from neurosarcoid participants coupled to T and B cell receptor sequencing. In contrast to pulmonary sarcoidosis, which is driven by CD4 T cells, we found CD8 T cell clonal expansion enriched in the neurosarcoid CSF. These CSF-enriched CD8 T cells were composed of two subsets with differential expression of EBI2, CXCR3, and CXCR4. Lastly, our data suggest that IFNγ signaling may distinguish neurosarcoidosis from other neurological disorders.
Keywords
Interferon, Neurosarcoidosis, Sarcoidosis, Single-cell RNA sequencing, T cells, TCR sequencing
Medical Subject Headings
CD4-Positive T-Lymphocytes (metabolism); CD8-Positive T-Lymphocytes (metabolism); Central Nervous System Diseases (cerebrospinal fluid); Humans; Sarcoidosis (cerebrospinal fluid)
Publication Date
6-15-2022
Publication Title
Journal of neuroimmunology
E-ISSN
1872-8421
Volume
367
First Page
577860
PubMed ID
35405431
Digital Object Identifier (DOI)
10.1016/j.jneuroim.2022.577860
Recommended Citation
Paley, Michael A.; Baker, Brandi J.; Dunham, S Richard; Linskey, Nicole; Cantoni, Claudia; Lee, Kenneth; Hassman, Lynn M.; Laurent, Jennifer; Roberson, Elisha D.; Clifford, David B.; and Yokoyama, Wayne M., "The CSF in neurosarcoidosis contains consistent clonal expansion of CD8 T cells, but not CD4 T cells" (2022). Translational Neuroscience. 2351.
https://scholar.barrowneuro.org/neurobiology/2351