MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers
Document Type
Article
Abstract
Identification of novel targets and biomarkers, such as microRNAs, is extremely helpful to understand the pathogenetic mechanisms in a disease like multiple sclerosis (MS). We tested the expression profile of 1145 microRNAs in peripheral blood mononuclear cells (PBMCs) of 19 MS patients and 14 controls, and we further explored their function by performing a whole-genome mRNA profiling in same subjects and using bioinformatic prediction tool. A total of 104 miRNAs have been identified as deregulated in MS patients; 2/10 which ranked highest (let-7g and miR-150) have been validated in a replication sample, leading to the identification of putative target genes.
Medical Subject Headings
Adult; Biomarkers (metabolism); Female; Gene Expression Profiling; Humans; Male; MicroRNAs (genetics, metabolism); Microarray Analysis; Middle Aged; Multiple Sclerosis (genetics); RNA, Messenger (metabolism)
Publication Date
2-2-2012
Publication Title
Neuroscience letters
E-ISSN
1872-7972
Volume
508
Issue
1
First Page
4
Last Page
8
PubMed ID
22108567
Digital Object Identifier (DOI)
10.1016/j.neulet.2011.11.006
Recommended Citation
Martinelli-Boneschi, Filippo; Fenoglio, Chiara; Brambilla, Paola; Sorosina, Melissa; Giacalone, Giacomo; Esposito, Federica; Serpente, Maria; Cantoni, Claudia; Ridolfi, Elisa; Rodegher, Mariaemma; Moiola, Lucia; Colombo, Bruno; De Riz, Milena; Martinelli, Vittorio; Scarpini, Elio; Comi, Giancarlo; and Galimberti, Daniela, "MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers" (2012). Translational Neuroscience. 2337.
https://scholar.barrowneuro.org/neurobiology/2337