Temporal pattern of C1q deposition after transient focal cerebral ischemia

Document Type

Article

Abstract

Recent studies have focused on elucidating the contribution of individual complement proteins to post-ischemic cellular injury. As the timing of complement activation and deposition after cerebral ischemia is not well understood, our study investigates the temporal pattern of C1q accumulation after experimental murine stroke. Brains were harvested from mice subjected to transient focal cerebral ischemia at 3, 6, 12, and 24 hr post reperfusion. Western blotting and light microscopy were employed to determine the temporal course of C1q protein accumulation and correlate this sequence with infarct evolution observed with TTC staining. Confocal microscopy was utilized to further characterize the cellular localization and characteristics of C1q deposition. Western Blot analysis showed that C1q protein begins to accumulate in the ischemic hemisphere between 3 and 6 hr post-ischemia. Light microscopy confirmed these findings, showing concurrent C1q protein staining of neurons. Confocal microscopy demonstrated co-localization of C1q protein with neuronal cell bodies as well as necrotic cellular debris. These experiments demonstrate the accumulation of C1q protein on neurons during the period of greatest infarct evolution. This data provides information regarding the optimal time window during which a potentially neuroprotective anti-C1q strategy is most likely to achieve therapeutic success.

Medical Subject Headings

Animals; Blotting, Western; Complement C1q (metabolism); Immunohistochemistry; Ischemic Attack, Transient (metabolism); Male; Mice; Mice, Inbred C57BL; Microscopy, Confocal; Neurons (metabolism); Time Factors

Publication Date

4-1-2006

Publication Title

Journal of neuroscience research

ISSN

0360-4012

Volume

83

Issue

5

First Page

883

Last Page

9

PubMed ID

16447284

Digital Object Identifier (DOI)

10.1002/jnr.20775

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