Isoflurane preconditioning affords functional neuroprotection in a murine model of intracerebral hemorrhage
Document Type
Article
Abstract
INTRODUCTION: Exposure to isoflurane gas prior to neurological injury, known as anesthetic preconditioning, has been shown to provide neuroprotective benefits in animal models of ischemic stroke. Given the common mediators of cellular injury in ischemic and hemorrhagic stroke, we hypothesize that isoflurane preconditioning will provide neurological protection in intracerebral hemorrhage (ICH). METHODS: 24 h prior to intracerebral hemorrhage, C57BL/6J mice were preconditioned with a 4-h exposure to 1% isoflurane gas or room air. Intracerebral hemorrhage was performed using a double infusion of 30-μL autologous whole blood. Neurological function was evaluated at 24, 48 and 72 h using the 28-point test. Mice were sacrificed at 72 h, and brain edema was measured. RESULTS: Mice preconditioned with isoflurane performed better than control mice on 28-point testing at 24 h, but not at 48 or 72 h. There was no significant difference in ipsilateral hemispheric edema between mice preconditioned with isoflurane and control mice. CONCLUSION: These results demonstrate the early functional neuroprotective effects of anesthetic preconditioning in ICH and suggest that methods of preconditioning that afford protection in ischemia may also provide protection in ICH.
Medical Subject Headings
Albumins (metabolism); Anesthetics, Inhalation (administration & dosage); Animals; Brain (metabolism); Brain Edema (etiology); Cerebral Hemorrhage (pathology, physiopathology, prevention & control); Disease Models, Animal; Drug Administration Schedule; Functional Laterality; Isoflurane (administration & dosage); Male; Mice; Mice, Inbred C57BL; Nervous System Diseases (etiology, prevention & control); Time Factors
Publication Date
1-1-2011
Publication Title
Acta neurochirurgica. Supplement
ISSN
0065-1419
Volume
111
First Page
141
Last Page
4
PubMed ID
21725745
Digital Object Identifier (DOI)
10.1007/978-3-7091-0693-8_23
Recommended Citation
Gigante, Paul R.; Appelboom, Geoffrey; Hwang, Brian Y.; Haque, Raqeeb M.; Yeh, Mason L.; Ducruet, Andrew F.; Kellner, Christopher P.; Gorski, Justin; Keesecker, Sarah E.; and Connolly, E Sander, "Isoflurane preconditioning affords functional neuroprotection in a murine model of intracerebral hemorrhage" (2011). Translational Neuroscience. 2117.
https://scholar.barrowneuro.org/neurobiology/2117