A mouse model of intracerebral hemorrhage using autologous blood infusion
Document Type
Article
Abstract
The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.
Medical Subject Headings
Animals; Basal Ganglia; Blood Transfusion, Autologous; Cerebral Hemorrhage (drug therapy, etiology); Disease Models, Animal; Infusion Pumps; Male; Mice; Mice, Inbred C57BL; Postoperative Care
Publication Date
1-1-2008
Publication Title
Nature protocols
E-ISSN
1750-2799
Volume
3
Issue
1
First Page
122
Last Page
8
PubMed ID
18193028
Digital Object Identifier (DOI)
10.1038/nprot.2007.513
Recommended Citation
Rynkowski, Michal A.; Kim, Grace H.; Komotar, Ricardo J.; Otten, Marc L.; Ducruet, Andrew F.; Zacharia, Brad E.; Kellner, Christopher P.; Hahn, David K.; Merkow, Maxwell B.; Garrett, Matthew C.; Starke, Robert M.; Cho, Byung-Moon; Sosunov, Sergei A.; and Connolly, E Sander, "A mouse model of intracerebral hemorrhage using autologous blood infusion" (2008). Translational Neuroscience. 2051.
https://scholar.barrowneuro.org/neurobiology/2051