A mouse model of intracerebral hemorrhage using autologous blood infusion

Document Type

Article

Abstract

The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

Medical Subject Headings

Animals; Basal Ganglia; Blood Transfusion, Autologous; Cerebral Hemorrhage (drug therapy, etiology); Disease Models, Animal; Infusion Pumps; Male; Mice; Mice, Inbred C57BL; Postoperative Care

Publication Date

1-1-2008

Publication Title

Nature protocols

E-ISSN

1750-2799

Volume

3

Issue

1

First Page

122

Last Page

8

PubMed ID

18193028

Digital Object Identifier (DOI)

10.1038/nprot.2007.513

This document is currently not available here.

Share

COinS