Three-dimensional representation and cortical projection topography of the nucleus basalis (Ch4) in the macaque: concurrent demonstration of choline acetyltransferase and retrograde transport with a stabilized tetramethylbenzidine method for horseradish peroxidase

Document Type

Article

Abstract

Ninety-six percent of the nucleus basalis neurons that project to the neocortex contain choline acetyltransferase. These projections from the cholinergic component of the nucleus basalis (Ch4) are topographically organized so that each cortical area receives most of its cholinergic input from a different Ch4 sector. The three-dimensional reconstruction of these sectors reveals the presence of a complex structure. A stabilization procedure that was used in these experiments maintains all the advantages of the tetramethylbenzidine method for horseradish peroxidase while eliminating the vulnerability of the reaction-product to high pH and dehydrating agents. © 1986.

Keywords

choline acetyltransferase, cholinergic projection, horseradish peroxidase, nucleus basalis, tetramethylbenzidine

Publication Date

3-5-1986

Publication Title

Brain Research

ISSN

00068993

Volume

367

Issue

1-2

First Page

301

Last Page

308

PubMed ID

3516304

Digital Object Identifier (DOI)

10.1016/0006-8993(86)91607-0

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