Shift in the ratio of three-repeat tau and four-repeat tau mRNAs in individual cholinergic basal forebrain neurons in mild cognitive impairment and Alzheimer's disease
Document Type
Article
Abstract
Molecular mechanisms underlying tauopathy remain undetermined. In the current study, single cell gene expression profiling was coupled with custom-designed cDNA array analysis to evaluate tau expression and other cytoskeletal elements within individual neuronal populations in patients with no cognitive impairment (NCI), mild cognitive impairment (MCI), and Alzheimer's disease (AD). Results revealed a shift in the ratio of three-repeat tau (3Rtau) to four-repeat tau (4Rtau) mRNAs within individual human cholinergic basal forebrain (CBF) neurons within nucleus basalis (NB) and CA1 hippocampal neurons during the progression of AD, but not during normal aging. A shift in 3Rtau to 4Rtau may precipitate a cascade of events in the selective vulnerability of neurons, ultimately leading to frank neurofibrillary tangle (NFT) formation in tauopathies including AD. © 2006 The Authors.
Keywords
Expression profile, Microarray, Mild cognitive impairment, Neurodegeneration, Nucleus basalis, RNA amplification
Publication Date
3-1-2006
Publication Title
Journal of Neurochemistry
ISSN
00223042
E-ISSN
14714159
Volume
96
Issue
5
First Page
1401
Last Page
1408
PubMed ID
16478530
Digital Object Identifier (DOI)
10.1111/j.1471-4159.2005.03641.x
Recommended Citation
Ginsberg, Stephen D.; Che, Shaoli; Counts, Scott E.; and Mufson, Elliott J., "Shift in the ratio of three-repeat tau and four-repeat tau mRNAs in individual cholinergic basal forebrain neurons in mild cognitive impairment and Alzheimer's disease" (2006). Translational Neuroscience. 1949.
https://scholar.barrowneuro.org/neurobiology/1949