An Isotopic Rubidium Ion Efflux Assay For The Functional Characterization Of Nicotinic Acetylcholine Receptors On Clonal Cell Lines

Department

neurobiology

Document Type

Article

Abstract

An isotopic rubidium ion efflux assay has been developed for the functional characterization of nicotinic acetylcholine receptors on cultured neurons. This assay first involves the intracellular sequestration of isotopic potassium ion analog by the ouabain-sensitive action of a sodium-potassium ATPase. Subsequently, the release of isotopic rubidium ion through nicotinic acetylcholine receptor-coupled monovalent cation channels is activated by application of nicotinic agonists. Specificity of receptor-mediated efflux is demonstrated by its sensitivity to blockade by nicotinic, but not muscarinic, antagonists. The time course of agonist-mediated efflux, within the temperal limitations of the assay, indicates a slow inactivation of receptor function on prolonged exposure to agonist. Dose-response profiles (i) have characteristic shapes for different nicotinic agonists, (ii) are described by three operationally defined parameters, and (iii) reflect different affinities of agonists for binding sites that control receptor activation and functional inhibition. The rubidium ion efflux assay provides fewer hazards but greater sensitivity and resolution than isotopic sodium or rubidium ion influx assays for functional nicotinic receptors. © 1988.

Publication Date

11-15-1988

Publication Title

Analytical Biochemistry

ISSN

00032697

Volume

175

Issue

1

First Page

212

Last Page

218

Digital Object Identifier (DOI)

10.1016/0003-2697(88)90380-6

This document is currently not available here.

Share

COinS