Decreased trkA gene expression within basal forebrain neurons in Alzheimer's disease

Document Type

Article

Abstract

In situ hybridization for TrkA mRNA was combined with quantitative optical densitometry to evaluate whether the expression of this gene is altered within cholinergic basal forebrain neurons (CBF) in Alzheimer's disease (AD). TrkA mRNA within individual nucleus basalis neurons was significantly reduced (66%) in AD cases relative to aged controls. Reverse transcription polymerase chain reaction quantitative analyses confirmed that TrkA mRNA levels decreased markedly in AD. In contrast, expression of the gene coding for the low affinity p75(NTR) was not significantly altered in AD relative to aged controls. These data indicate that there is a selective defect in trkA gene expression in AD, supporting the hypothesis that the degeneration of CBF neurons seen in this disease results from impaired nerve growth factor trophic support.

Keywords

Ageing, Disease, Gene, Human, In situ hybridization, Receptors, TrkA

Publication Date

1-1-1997

Publication Title

NeuroReport

ISSN

09594965

Volume

8

Issue

1

First Page

25

Last Page

29

PubMed ID

9051746

Digital Object Identifier (DOI)

10.1097/00001756-199612200-00006

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