Apolipoprotein E-immunoreactivity in aged rhesus monkey cortex: Colocalization with amyloid plaques

Document Type

Article

Abstract

In the present study, we examined the relationship between ApoE and amyloid containing profiles within the cerebral cortex of young, middle aged, and aged Rhesus monkeys. Polymerase chain reaction analysis revealed a pattern consistent with the ApoE e4 phenotype in the rhesus monkey similar to that reported in humans. We found numerous ApoE immunoreactive plaques within the temporal neocortex and amygdala, whereas the hippocampus contained only a few such plaques. Although virtually all ApoE-immunoreactive plaques coexpressed β-amyloid, most plaques were βA4 positive/ApoE immunonegative within the aged monkey cortex. Moreover, we observed a close correspondence between ApoE and thioflavin-positive (i.e., amyloid) plaques suggesting that ApoE may play a critical role in the conversion of βA4 to its β-pleated form. Because ApoE, βA4 and amyloid are expressed in plaques within the aged Rhesus macaque cortex, this species may provide an in vivo model for investigations aimed at clarifying the interactions between these proteins in normal and pathologic aging. © 1994.

Keywords

Aging, Alzheimer's disease, Amyloid, Animal model, Antibodies, ApoE, Apolipoprotein, Immunohistochemistry, Monkey, Pathologic, Plaques, Thioflavin-S

Publication Date

1-1-1994

Publication Title

Neurobiology of Aging

ISSN

01974580

Volume

15

Issue

5

First Page

621

Last Page

627

PubMed ID

7824054

Digital Object Identifier (DOI)

10.1016/0197-4580(94)00064-6

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