Mapping Single-Cell-Substrate Interactions By Surface Plasmon Resonance Microscopy
We report the imaging of the cell-substrate adhesion of a single cell with subcellular spatial resolution. Osmotic pressure was introduced to provide a controllable mechanical stimulation to the cell attached to a substrate, and high-resolution surface plasmon resonance microscopy was used to map the response of the cell, from which local cell-substrate adhesion was determined. In addition to high spatial resolution, the approach is noninvasive and fast and allows for the continuous mapping of cell-substrate interactions and single-cell movements. Â© 2012 American Chemical Society.
Digital Object Identifier (DOI)
Wang, Wei; Wang, Shaopeng; Liu, Qiang; Wu, Jie; and Tao, Nongjian, "Mapping Single-Cell-Substrate Interactions By Surface Plasmon Resonance Microscopy" (2012). Translational Neuroscience. 159.