Engineered Agrin Attenuates The Severity Of Experimental Autoimmune Myasthenia Gravis
Department
neurobiology
Document Type
Article
Abstract
Introduction: Agrin is essential for the formation and maintenance of neuromuscular junctions (NMJs). NT-1654 is a C-terminal fragment of mouse neural agrin. In this study, we determined the effects of NT-1654 on the severity of experimental autoimmune myasthenia gravis (EAMG). Methods: EAMG was induced in female Lewis rats by immunization with the Torpedo acetylcholine receptor (tAChR) and complete Freund's adjuvant (CFA). NT-1654 was dissolved in phosphate-buffered saline (PBS) and injected daily subcutaneously into tAChR immunized rats during the first 10 days after immunization, and then every other day for the following 20 days. Results: We showed that NT-1654 attenuated clinical severity, effectively promoted the clustering of AChRs at NMJs, and alleviated the impairment of NMJ transmission and the reduction of muscle-specific kinase (MuSK) in EAMG rats. Discussion: We demonstrated that NT-1654 attenuated clinical severity, effectively promoted the clustering of AChRs at NMJs, and alleviated the impairment of NMJ transmission and the reduction of muscle-specific kinase (MuSK) in EAMG rats. Muscle Nerve 57: 814–820, 2018.
Publication Date
5-1-2018
Publication Title
Muscle and Nerve
ISSN
0148639X
Volume
57
Issue
5
First Page
814
Last Page
820
Digital Object Identifier (DOI)
10.1002/mus.26025
Recommended Citation
Li, Zhiguo; Li, Minshu; Wood, Kristofer; Hettwer, Steffan; Muley, Suraj A.; Shi, Fu Dong; Liu, Qiang; and Ladha, Shafeeq S., "Engineered Agrin Attenuates The Severity Of Experimental Autoimmune Myasthenia Gravis" (2018). Translational Neuroscience. 140.
https://scholar.barrowneuro.org/neurobiology/140