Tau aggregates are RNA-protein assemblies that mislocalize multiple nuclear speckle components

Document Type

Article

Abstract

Tau aggregates contribute to neurodegenerative diseases, including frontotemporal dementia and Alzheimer's disease (AD). Although RNA promotes tau aggregation in vitro, whether tau aggregates in cells contain RNA is unknown. We demonstrate, in cell culture and mouse brains, that cytosolic and nuclear tau aggregates contain RNA with enrichment for small nuclear RNAs (snRNAs) and small nucleolar RNAs (snoRNAs). Nuclear tau aggregates colocalize with and alter the composition, dynamics, and organization of nuclear speckles, membraneless organelles involved in pre-mRNA splicing. Moreover, several nuclear speckle components, including SRRM2, mislocalize to cytosolic tau aggregates in cells, mouse brains, and brains of individuals with AD, frontotemporal dementia (FTD), and corticobasal degeneration (CBD). Consistent with these alterations, we observe that the presence of tau aggregates is sufficient to alter pre-mRNA splicing. This work identifies tau alteration of nuclear speckles as a feature of tau aggregation that may contribute to the pathology of tau aggregates.

Keywords

Alzheimer's Disease, Frontemporal Dementia, MAPT, Nuclear Speckles, RNA, RNA binding proteins, snRNA, Splicing, SRRM2, Tau

Publication Date

5-19-2021

Publication Title

Neuron

ISSN

08966273

E-ISSN

10974199

Volume

109

Issue

10

First Page

1675

Last Page

1691.e9

PubMed ID

33848474

Digital Object Identifier (DOI)

10.1016/j.neuron.2021.03.026

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