The neuronal Rho-GEF Kalirin-7 interacts with PDZ domain-containing proteins and regulates dendritic morphogenesis
Document Type
Article
Abstract
Spine function requires precise control of the actin cytoskeleton. Kalirin-7, a GDP/GTP exchange factor for Rac1, interacts with PDZ proteins such as PSD-95, colocalizing with PSD-95 at synapses of cultured hippocampal neurons. PSD-95 and Kalirin-7 interact in vivo and in heterologous expression systems. In primary cortical neurons, transfected Kalirin-7 is targeted to spines and increases the number and size of spine-like structures. A Kalirin-7 mutant unable to interact with PDZ proteins remains in the cell soma, inducing local formation of aberrant filopodial neurites. Kalirin-7 with an inactivated GEF domain reduces the number of spines below control levels. These results provide evidence that PDZ proteins target Kalirin-7 to the PSD, where it regulates dendritic morphogenesis through Rac1 signaling to the actin cytoskeleton.
Publication Date
1-1-2001
Publication Title
Neuron
ISSN
08966273
Volume
29
Issue
1
First Page
229
Last Page
242
PubMed ID
11182094
Digital Object Identifier (DOI)
10.1016/S0896-6273(01)00193-3
Recommended Citation
Penzes, Peter; Johnson, Richard C.; Sattler, Rita; Zhang, Xiaoqun; Huganir, Richard L.; Kambampati, Vikram; Mains, Richard E.; and Eipper, Betty A., "The neuronal Rho-GEF Kalirin-7 interacts with PDZ domain-containing proteins and regulates dendritic morphogenesis" (2001). Translational Neuroscience. 1379.
https://scholar.barrowneuro.org/neurobiology/1379