GluR1 controls dendrite growth through its binding partner, SAP97
Document Type
Article
Abstract
Activity-dependent dendrite elaboration influences the pattern of interneuronal connectivity and network function. In the present study, we examined the mechanism by which the GluR1 subunit of AMPA receptors controls dendrite morphogenesis. GluR1 binds to SAP97, a scaffolding protein that is a component of the postsynaptic density, via its C-terminal 7 aa. We find that elimination of this interaction in vitro or in vivo (by deleting the C-terminal 7 aa of GluR1, GluR1Delta7) does not influence trafficking, processing, or cell surface GluR1 expression but does prevent translocation of SAP97 from the cytosol to membranes. GluR1 and SAP97 together at the plasma membrane promotes dendrite branching in an activity-dependent manner, although this does not require physical association. Our findings suggest that the C-terminal 7 aa of GluR1 are essential for bringing SAP97 to the plasma membrane, where it acts to translate the activity of AMPA receptors into dendrite growth.
Medical Subject Headings
Adaptor Proteins, Signal Transducing (metabolism); Animals; Cell Membrane (metabolism); Cells, Cultured; Chimera; Dendrites (metabolism, physiology); Discs Large Homolog 1 Protein; Membrane Proteins (metabolism); Mice; Mice, Inbred C57BL; Mice, Mutant Strains; Motor Neurons (metabolism); Protein Transport (physiology); Receptors, AMPA (metabolism); Spinal Cord (physiology, ultrastructure)
Publication Date
10-8-2008
Publication Title
The Journal of neuroscience : the official journal of the Society for Neuroscience
E-ISSN
1529-2401
Volume
28
Issue
41
First Page
10220
Last Page
33
PubMed ID
18842882
Digital Object Identifier (DOI)
10.1523/JNEUROSCI.3434-08.2008
Recommended Citation
Zhou, Weiguo; Zhang, Lei; Guoxiang, Xiong; Mojsilovic-Petrovic, Jelena; Takamaya, Kogo; Sattler, Rita; Huganir, Richard; and Kalb, Robert, "GluR1 controls dendrite growth through its binding partner, SAP97" (2008). Translational Neuroscience. 1364.
https://scholar.barrowneuro.org/neurobiology/1364