Manganese-based superoxide dismutase mimics modify both acute and long-term outcome severity in a Drosophila melanogaster model of classic galactosemia
Document Type
Article
Abstract
Aims: The goal of this study was to use two manganese (Mn)-based superoxide dismutase (SOD) mimics to test the hypothesis that reactive oxygen species contribute to both acute and long-term outcomes in a galactose-1P uridylyltransferase (GALT)-null Drosophila melanogaster model of classic galactosemia. Results: We tested the impact of each of two Mn porphyrin SOD mimics, MnTnBuOE-2-PyP5+, and MnTE-2-PyP5+, (i) on survival of GALT-null Drosophila larvae reared in the presence versus absence of dietary galactose and (ii) on the severity of a long-term movement defect in GALT-null adult flies. Both SOD mimics conferred a significant survival benefit to GALT-null larvae exposed to galactose but not to controls or to GALT-null larvae reared in the absence of galactose. One mimic, MnTE-2-PyP5+, also largely rescued a galactose-independent long-term movement defect otherwise seen in adult GALT-null flies. The survival benefit of both SOD mimics occurred despite continued accumulation of elevated galactose-1P in the treated animals, and studies of thiolated proteins demonstrated that in both the presence and absence of dietary galactose MnTE-2-PyP5+ largely prevented the elevated protein oxidative damage otherwise seen in GALT-null animals relative to controls. Innovation and Conclusions: Our results confirm oxidative stress as a mediator of acute galactose sensitivity in GALT-null Drosophila larvae and demonstrate for the first time that oxidative stress may also contribute to galactose-independent adult outcomes in GALT deficiency. Finally, our results demonstrate for the first time that both MnTnBuOE-2-PyP5+ and MnTE-2-PyP5+ are bioavailable and effective when administered through an oral route in a D. melanogaster model of classic galactosemia. Antioxid. Redox Signal. 20, 2361-2371. © Copyright 2014, Mary Ann Liebert, Inc. 2014.
Publication Date
5-20-2014
Publication Title
Antioxidants and Redox Signaling
ISSN
15230864
E-ISSN
15577716
Volume
20
Issue
15
First Page
2361
Last Page
2371
PubMed ID
23758052
Digital Object Identifier (DOI)
10.1089/ars.2012.5122
Recommended Citation
Jumbo-Lucioni, Patricia P.; Ryan, Emily L.; Hopson, Marquise L.; Bishop, Heather M.; Weitner, Tin; Tovmasyan, Artak; Spasojevic, Ivan; Batinic-Haberle, Ines; Liang, Yongliang; Jones, Dean P.; and Fridovich-Keil, Judith L., "Manganese-based superoxide dismutase mimics modify both acute and long-term outcome severity in a Drosophila melanogaster model of classic galactosemia" (2014). Translational Neuroscience. 1302.
https://scholar.barrowneuro.org/neurobiology/1302