Title

Miniaturized handheld confocal microscopy for neurosurgery: results in an experimental glioblastoma model.

Department

Neurosurgery; Neuropathology; Research

Document Type

Article

Abstract

INTRODUCTION: Recent developments in optical science and image processing have miniaturized the components required for confocal microscopy. Clinical confocal imaging applications have emerged, including assessment of colonic mucosal dysplasia during colonoscopy. We present our initial experience with handheld, miniaturized confocal imaging in a murine brain tumor model.

METHODS: Twelve C57/BL6 mice were implanted intracranially with 10(5) GL261 glioblastoma cells. The brains of 6 anesthetized mice each at 14 and 21 days after implantation were exposed surgically, and the brain surface was imaged using a handheld confocal probe affixed to a stereotactic frame. The probe was moved systematically over regions of normal and tumor-containing tissue. Intravenous fluorescein and topical acriflavine contrast agents were used. Biopsies were obtained at each imaging site beneath the probe and assessed histologically. Mice were killed after imaging.

RESULTS: Handheld confocal imaging produced exquisite images, well-correlated with corresponding histologic sections, of cellular shape and tissue architecture in murine brain infiltrated by glial neoplasm. Reproducible patterns of cortical vasculature, as well as normal gray and white matter, were identified. Imaging effectively distinguished between tumor and nontumor tissue, including infiltrative tumor margins. Margins were easily identified by observers without prior neuropathology training after minimum experience with the technology.

CONCLUSION: Miniaturized handheld confocal imaging may assist neurosurgeons in detecting infiltrative brain tumor margins during surgery. It may help to avoid sampling error during biopsy of heterogeneous glial neoplasms, with the potential to supplement conventional intraoperative frozen section pathology. Clinical trials are warranted on the basis of these promising initial results.

Medical Subject Headings

Animals; Brain Neoplasms; Cell Line, Tumor; Disease Models, Animal; Ergonomics; Female; Glioblastoma; Hand; Humans; Image Processing, Computer-Assisted; Mice; Mice, Inbred C57BL; Microscopy, Confocal; Neurosurgery; Optical Fibers

Publication Date

2-1-2010

Publication Title

Neurosurgery

ISSN

1524-4040

Volume

66

Issue

2

First Page

410

Last Page

417

PubMed ID

20087141

Digital Object Identifier (DOI)

10.1227/01.NEU.0000365772.66324.6F

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