Drosophila homolog of the murine Int-1 protooncogene.

Document Type

Article

Abstract

We have isolated phage clones from Drosophila melanogaster genomic and cDNA libraries containing a sequence homologous to the murine Int-1 protooncogene. The Drosophila gene is represented by a single locus at position 28A1-2 on chromosome 2. The gene is expressed as a 2.9-kilobase-long polyadenylylated mRNA in embryo, larval, and pupal stages. It is hardly detectable in adult flies. The longest open reading frame of the cDNA clone corresponds to a protein 469 amino acids long. Alignment of the predicted amino acid sequences shows that the Drosophila protein is 86 amino acids longer than its murine counterpart. In spite of the difference in length, the two proteins are highly conserved with an overall sequence homology of 54%. Both Drosophila and murine Int-1 proteins begin with a hydrophobic leader sequence and contain cysteine residues and sites for glycosylation (four in the murine protein and one in the Drosophila protein) in conserved positions, suggesting that they play important functional roles.

Medical Subject Headings

Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping; Cloning, Molecular; Drosophila melanogaster; Mice; Molecular Sequence Data; Nucleic Acid Hybridization; Proto-Oncogenes; RNA, Messenger

Publication Date

5-1-1988

Publication Title

Proceedings of the National Academy of Sciences of the United States of America

ISSN

0027-8424

Volume

85

Issue

9

First Page

3034

Last Page

3038

PubMed ID

3129722

Digital Object Identifier (DOI)

10.1073/pnas.85.9.3034

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