Multivariate analysis of differential lymphocyte cell cycle activity in Alzheimer's disease

Document Type

Article

Abstract

Mounting evidence suggests cell cycle dysregulation is involved in the pathogenesis of Alzheimer's disease (AD) and that this failure is systemic, affecting not only neurons but also peripheral blood lymphocytes (PBLs). This study analyzed if differences in PBL proliferation activity could be used as a diagnostic biomarker for AD. CD69 and CD28 expressions on PBL T, B, and monocyte cells were measured by flow cytometry with and without mitogenic stimulation in healthy controls (HC), probable AD, and Parkinson's disease dementia (PDD) subjects. Univariate and multivariate scoring models were employed to evaluate the data relative to the clinical diagnoses. Eleven CD expression markers were significantly altered in AD subjects compared with a mixed pool of PDD and HC subjects using univariate models. Using multivariate models, seven CD expression markers were significantly altered in AD subjects compared with PDD subjects. Multivariate scoring demonstrated up to a 91% positive and 92% negative agreement with subject clinical diagnosis and had little correlation with the severity of dementia. Present findings suggest that with further development this analytical and multivariate modeling procedure could aid the current differential diagnosis of Alzheimer's disease. © 2012 Elsevier Inc.

Publication Date

2-1-2012

Publication Title

Neurobiology of Aging

ISSN

01974580

E-ISSN

15581497

Volume

33

Issue

2

First Page

234

Last Page

241

PubMed ID

20395014

Digital Object Identifier (DOI)

10.1016/j.neurobiolaging.2010.03.001

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