Title

Generation of mice with a conditional and reporter allele for Tmem100

Document Type

Article

Abstract

Activin receptor-like kinase 1 (ACVRL1; ALK1) is predominantly expressed in arterial endothelial cells and plays an important role in angiogenesis. ACVRL1 mutations cause hereditary hemorrhagic telangiectasia (HHT), a genetic vascular disorder for which the underlying mechanism is poorly understood. We have found that expression of transmembrane protein 100 (Tmem100) is downregulated in the lung of Acvrl1-deficient mice; however, its function is unknown. To elucidate the role of Tmem100 in vivo, we generated a conditional knockout allele for Tmem100 in which exon3, containing the entire coding sequence, was flanked by loxP sequences. The targeted allele also possessed a lacZ reporter cassette in intron2 for visualization of Tmem100 expression. We found that Tmem100 was predominantly expressed in arterial endothelial cells of developing embryos. The conditional and reporter allele will be a useful resource to investigate the in vivo role of Tmem100, especially in angiogenesis. © 2010 Wiley-Liss, Inc.

Publication Date

11-1-2010

Publication Title

Genesis

ISSN

1526954X

E-ISSN

1526968X

Volume

48

Issue

11

First Page

673

Last Page

678

PubMed ID

20848592

Digital Object Identifier (DOI)

10.1002/dvg.20674

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