Title

SEC6 encodes an 85 kDa soluble protein required for exocytosis in yeast

Document Type

Article

Abstract

The SEC6 gene encodes a protein required for an event leading to fusion of post‐Golgi vesicles with the plasma membrane in Saccharomyces cerevisiae cells. The gene was cloned by complementation of the temperature‐sensitive growth defect of a sec6‐4 strain. The nucleotide sequence was determined and the longest open reading frame was found to encode an 85 kDa protein of 733 amino acids. The Sec6 protein is predicted to be hydrophilic and is found predominantly in the soluble fraction of a yeast lysate, in a species that sediments with a coefficient of 14S. No extnsive homology was found with known proteins of the database. Gene disruption and marker rescue experiments indicate that SEC6 is a single copy gene essential for growth. Overproduction of Sec6p does not suppress any of the other lateactind sec mutants, yet sec6‐4 does display synthetic lethality with sec8‐9, suggesting that the two products may fulfill inter‐related functions. Copyright © 1992 John Wiley & Sons Ltd.

Publication Date

1-1-1992

Publication Title

Yeast

ISSN

0749503X

E-ISSN

10970061

Volume

8

Issue

7

First Page

549

Last Page

558

PubMed ID

1523887

Digital Object Identifier (DOI)

10.1002/yea.320080706

This document is currently not available here.

Share

COinS