Background/Aims: Although some evidence suggests that the prevalence of osteoarthritis (OA) is lower in smokers compared to nonsmokers, the mechanisms of nicotine-induced protection remain unclear. Stimulation of the Î±7 nicotinic acetylcholine receptor (Î±7-nAChR) appears to be a critical mechanism underlying the anti-inflammatory potential of cholinergic agonists in immune cells. The inhibition of secreted inflammatory molecules and the subsequent inflammatory processes have been proposed as a novel strategy for the treatment of OA. The objective of the present study was to determine whether nicotine-induced protection in a monosodium iodoacetate (MIA) rat model of OA occurs via Î±7-nAChR-mediated inhibition of chondrocytes. Methods: Both in vivo (MIA) and in vitro (MIA; Interleukin-1Î², IL-1Î²) models of OA were used to investigate the roles and the possible mechanisms whereby Î±7-nAChRs protect against knee joint degradation. Multiple experimental approaches, including macroscopic, histological analysis, chondrocyte cell cultures, confocal microscopy, and western blotting, were employed to elucidate the mechanisms of Î±7-nAChR-mediated protection. Results: Systemic administration of nicotine alleviated MIA-induced joint degradation. The protective effects of nicotine were abolished by administration of the Î±7-nAChR-selective antagonist methyllycaconitine (MLA). In primary cultured rat chondrocytes, pretreatment with nicotine suppressed both p38, extracellular regulated kinase (Erk) 1/2 and c-Jun-N-terminal kinase (JNK) mitogen-activated protein kinases (MAPK) phosphorylation and phosphorylated nuclear factor-kappa B (NF-ÎºB) p65 activation induced by MIA- or IL-1Î², and these effects were also reversed by MLA. Conclusion: Taken together, our results suggest that activation Î±7-nAChRs is an important mechanism underlying the protective effects of nicotine.
Cellular Physiology and Biochemistry
Digital Object Identifier (DOI)
Liu, Yuan; Wu, Dongying; Song, Fanglong; Zhu, Chenlei; Hui, Yujian; Zhu, Qingcheng; Wu, Jie; Fan, Weimin; and Hu, Jun, "Activation Of Î±7 Nicotinic Acetylcholine Receptors Prevents Monosodium Iodoacetate-Induced Osteoarthritis In Rats" (2015). Neurobiology. 403.