2-Aminoethoxydiphenyl Borate Inhibits Agonist-Induced Ca2+ Signals By Blocking Inositol Trisphosphate Formation In Acutely Dissociated Mouse Pancreatic Acinar Cells
Evidence suggests that 2-aminoethoxydiphenyl borate (2-APB) modulates intracellular Ca2+ signals in a complex manner. 2-APB inhibits or potentiates intracellular Ca2+ signals in different cell types, perhaps through different mechanisms. Here, we report a novel mechanism underlying 2-APB-induced inhibition of agonist-activated Ca2+ oscillations in mouse pancreatic acinar cells, using patch-clamp and biochemical techniques. Pre-treatment of the cells with 100 Î¼M 2-APB completely abolished ACh-but not inositol trisphosphate (InsP3)-induced Ca 2+ oscillations, suggesting that the mechanism of inhibition occurs between cytoplasmic receptors and InsP3 receptor activation. In addition, 100 Î¼M 2-APB significantly inhibited ACh-induced phospholipase C (PLC) activation. These findings indicate that, in mouse pancreatic acinar cells, in addition to modulating InsP3 receptors and blocking the store-operated Ca2+ pathway, high concentrations of 2-APB also inhibit agonist-induced Ca2+ signals by reducing InsP3 formation.
Pflugers Archiv European Journal of Physiology
Digital Object Identifier (DOI)
Wu, Jie; Takeo, Teruko; Suga, Sechiko; Kanno, Takahiro; Osanai, Tomohiro; Mikoshiba, Katsuhiko; and Wakui, Makoto, "2-Aminoethoxydiphenyl Borate Inhibits Agonist-Induced Ca2+ Signals By Blocking Inositol Trisphosphate Formation In Acutely Dissociated Mouse Pancreatic Acinar Cells" (2004). Translational Neuroscience. 398.