2-Aminoethoxydiphenyl Borate Inhibits Agonist-Induced Ca2+ Signals By Blocking Inositol Trisphosphate Formation In Acutely Dissociated Mouse Pancreatic Acinar Cells

Department

neurobiology

Document Type

Article

Abstract

Evidence suggests that 2-aminoethoxydiphenyl borate (2-APB) modulates intracellular Ca2+ signals in a complex manner. 2-APB inhibits or potentiates intracellular Ca2+ signals in different cell types, perhaps through different mechanisms. Here, we report a novel mechanism underlying 2-APB-induced inhibition of agonist-activated Ca2+ oscillations in mouse pancreatic acinar cells, using patch-clamp and biochemical techniques. Pre-treatment of the cells with 100 μM 2-APB completely abolished ACh-but not inositol trisphosphate (InsP3)-induced Ca 2+ oscillations, suggesting that the mechanism of inhibition occurs between cytoplasmic receptors and InsP3 receptor activation. In addition, 100 μM 2-APB significantly inhibited ACh-induced phospholipase C (PLC) activation. These findings indicate that, in mouse pancreatic acinar cells, in addition to modulating InsP3 receptors and blocking the store-operated Ca2+ pathway, high concentrations of 2-APB also inhibit agonist-induced Ca2+ signals by reducing InsP3 formation.

Publication Date

9-1-2004

Publication Title

Pflugers Archiv European Journal of Physiology

ISSN

00316768

Volume

448

Issue

6

First Page

592

Last Page

595

Digital Object Identifier (DOI)

10.1007/s00424-004-1295-0

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