Nicotinic Acetylcholine Receptors On Nt2 Precursor Cells And Hnt (Nt2-N) Neurons
This is the first report, to our knowledge, of prominent, natural expression of nAChR Î±4, Î±6 and Î±9 subunits in a human, neuronally-committed cell line. We performed studies with specific reference to the expression of nicotinic acetylcholine receptors (nAChR) to further characterize a human, postmitotic, transplantable, with a neuronal phenotype, cell line called hNT (also called NT2-N). hNT cells acquire a distinctive neuronal phenotype upon differentiation from their NT2 precursors. Immunocytochemical studies showed that NT2 cells were strongly immunopositive for Î±4 or Î±7 subunits, moderately immunopositive for Î±3/Î±5 subunits, and weakly immunopositive for Î²2 or Î²4 subunits, whereas hNT neurons showed positive, strong-to-moderate immunostaining for all of these nAChR subunits. Reverse transcription-polymerase chain reaction (RT-PCR) mRNA analyses indicated that levels of Î±7 subunit messages were similar in both NT2 and hNT cells, whereas Î±2, Î±10, and Î²3 subunit transcripts were not detected. Levels of Î±3, Î±5, and Î²4 subunit messages were lower in hNT neurons than in NT2 precursors. However, Î±4 and Î²2 subunit messages were present in NT2 precursors but were greatly induced in hNT neurons. Levels of Î±6 and Î±9 subunit messages, not detectable in NT2 precursors, rose to high levels in hNT neurons. hNT cell nAChR subunit message levels were comparable to (Î±4, Î±5, Î²4) or higher than (Î±6, Î±9, Î²2) levels in adult human brain. NT2 and hNT cells may provide an excellent model for studies of neurogenesis, roles played by nAChR in differentiation and neurodegeneration, and effects of neuronal differentiation on nAChR expression. Â© 2002 Elsevier Science B.V. All rights reserved.
Developmental Brain Research
Digital Object Identifier (DOI)
Newman, Mary B.; Kuo, Yen Ping; Lukas, Ronald J.; Sanberg, Paul R.; Shytle, R. Douglas; McGrogan, Michael P.; and Zigova, Tanja, "Nicotinic Acetylcholine Receptors On Nt2 Precursor Cells And Hnt (Nt2-N) Neurons" (2002). Translational Neuroscience. 258.