Title

Quantifying the mechanical and histological properties of thrombus analog made from human blood for the creation of synthetic thrombus for thrombectomy device testing

Document Type

Article

Abstract

BACKGROUND: Untreated ischemic stroke can lead to severe morbidity and death, and as such, there are numerous endovascular blood-clot removal (thrombectomy) devices approved for human use. Human thrombi types are highly variable and are typically classified in qualitative terms - 'soft/red,' 'hard/white,' or 'aged/calcified.' Quantifying human thrombus properties can accelerate the development of thrombus analogs for the study of thrombectomy outcomes, which are often inconsistent among treated patients. METHODS: 'Soft'human thrombi were created from blood samples ex vivo (ie, human blood clotted in sample vials) and tested for mechanical properties using a hybrid rheometer material testing system. Synthetic thrombus materials were also mechanically tested and compared with the 'soft' human blood clots. RESULTS: Mechanical testing quantified the shear modulus and dynamic (elastic) modulus of volunteer human thrombus samples. This data was used to formulate a synthetic blood clot made from a composite polymer hydrogel of polyacrylamide and alginate (PAAM-Alg). The PAAM-Alg interpenetrating network of covalently and ionically cross-linked polymers had tunable elastic and shear moduli properties and shape memory characteristics. CONCLUSIONS: Due to its adjustable properties, PAAM-Alg can be modified to mimic various thrombi classifications. Future studies will include obtaining and quantitatively classifying patient thrombectomy samples and altering the PAAM-Alg to mimic the results for use with in vitro thrombectomy studies.

Keywords

device, stroke, thrombectomy

Medical Subject Headings

Adult; Blood Physiological Phenomena; Elasticity (physiology); Female; Humans; Male; Materials Testing (methods); Rheology (instrumentation, methods); Shear Strength (physiology); Thrombectomy (instrumentation, methods); Thrombosis (diagnosis, physiopathology); Young Adult

Publication Date

12-1-2018

Publication Title

Journal of neurointerventional surgery

E-ISSN

1759-8486

Volume

10

Issue

12

First Page

1168

Last Page

1173

PubMed ID

29695602

Digital Object Identifier (DOI)

10.1136/neurintsurg-2017-013675

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