Characterization Of Nicotinic Acetylcholine Receptor Channels Of The Te671 Human Medulloblastoma Clonal Line

Authors

Robert E. Oswald
Roger L. Papke
Ronald J. Lukas, Barrow Neurological InstituteFollow

Department

neurobiology

Document Type

Article

Abstract

Acetylcholine (ACh)-gated single channel events were studied on the TE671 human medulloblastoma clonal cell line by the use of the cell-attached patch clamp technique. Channel activity was detected (86% probability) in the presence of 0.1 2 μM ACh but not (0% probability) in the absence of agonist or in the presence of 1 μM α-bungarotoxin (Bgt). This effect of Bgt was reversible within 1 h. The most prominent channel type had a conductance of 50 pS. The kinetics of opening and closing of the channel were similar to that for skeletal muscle nicotinic acetylcholine receptors. © 1989.

Publication Date

1-16-1989

Publication Title

Neuroscience Letters

ISSN

03043940

Volume

96

Issue

2

First Page

207

Last Page

212

Digital Object Identifier (DOI)

10.1016/0304-3940(89)90059-1

Recommended Citation

Oswald, Robert E.; Papke, Roger L.; and Lukas, Ronald J., "Characterization Of Nicotinic Acetylcholine Receptor Channels Of The Te671 Human Medulloblastoma Clonal Line" (1989). Translational Neuroscience. 193.
https://scholar.barrowneuro.org/neurobiology/193