Characterization Of Nicotinic Acetylcholine Receptor Channels Of The Te671 Human Medulloblastoma Clonal Line
Acetylcholine (ACh)-gated single channel events were studied on the TE671 human medulloblastoma clonal cell line by the use of the cell-attached patch clamp technique. Channel activity was detected (86% probability) in the presence of 0.1 2 Î¼M ACh but not (0% probability) in the absence of agonist or in the presence of 1 Î¼M Î±-bungarotoxin (Bgt). This effect of Bgt was reversible within 1 h. The most prominent channel type had a conductance of 50 pS. The kinetics of opening and closing of the channel were similar to that for skeletal muscle nicotinic acetylcholine receptors. Â© 1989.
Digital Object Identifier (DOI)
Oswald, Robert E.; Papke, Roger L.; and Lukas, Ronald J., "Characterization Of Nicotinic Acetylcholine Receptor Channels Of The Te671 Human Medulloblastoma Clonal Line" (1989). Translational Neuroscience. 193.