Non-Invasive Tracking Of Cd4 + T Cells With A Paramagnetic And Fluorescent Nanoparticle In Brain Ischemia
Recent studies have demonstrated that lymphocytes play a key role in ischemic brain injury. However, there is still a lack of viable approaches to non-invasively track infiltrating lymphocytes and reveal their key spatiotemporal events in the inflamed central nervous system (CNS). Here we describe an in vivo imaging approach for sequential monitoring of brain-infiltrating CD4 + T cells in experimental ischemic stroke. We show that magnetic resonance imaging (MRI) or Xenogen imaging combined with labeling of SPIO-Molday ION Rhodamine-B (MIRB) can be used to monitor the dynamics of CD4 + T cells in a passive transfer model. MIRB-labeled CD4 + T cells can be longitudinally visualized in the mouse brain and peripheral organs such as the spleen and liver after cerebral ischemia. Immunostaining of tissue sections showed similar kinetics of MIRB-labeled CD4 + T cells when compared with in vivo observations. Our results demonstrated the use of MIRB coupled with in vivo imaging as a valid method to track CD4 + T cells in ischemic brain injury. This approach will facilitate future investigations to identify the dynamics and key spatiotemporal events for brain-infiltrating lymphocytes in CNS inflammatory diseases.
Journal of Cerebral Blood Flow and Metabolism
Digital Object Identifier (DOI)
Jin, Wei Na; Yang, Xiaoxia; Li, Zhiguo; Li, Minshu; Yu, Samuel Xiang; Wood, Kristofer; Liu, Qingwei; Fu, Ying; Han, Wei; Xu, Yun; Shi, Fu Dong; and Liu, Qiang, "Non-Invasive Tracking Of Cd4 + T Cells With A Paramagnetic And Fluorescent Nanoparticle In Brain Ischemia" (2016). Translational Neuroscience. 165.