Title

Intraparenchymal stereotaxic delivery of rAAV and special considerations in vector handling

Document Type

Article

Abstract

Stereotaxic surgery enables precise and consistent microinjections to discrete neural nuclei. Using stereotaxic surgery to deliver viral vectors is a powerful tool that provides the ability to manipulate gene expression in specific regions, or even specific cell types in the brain. Here, we describe the proper handling and stereotaxic delivery of recombinant adeno-associated virus to various neuroanatomical structures of the rodent brain.

Keywords

Adeno-associated virus (rAAV), Brain, Gene delivery, Stereotaxic surgery

Publication Date

1-1-2016

Publication Title

Methods in Molecular Biology

ISSN

10643745

Volume

1382

First Page

199

Last Page

215

PubMed ID

26611588

Digital Object Identifier (DOI)

10.1007/978-1-4939-3271-9_14

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