Intraparenchymal stereotaxic delivery of rAAV and special considerations in vector handling
Document Type
Article
Abstract
Stereotaxic surgery enables precise and consistent microinjections to discrete neural nuclei. Using stereotaxic surgery to deliver viral vectors is a powerful tool that provides the ability to manipulate gene expression in specific regions, or even specific cell types in the brain. Here, we describe the proper handling and stereotaxic delivery of recombinant adeno-associated virus to various neuroanatomical structures of the rodent brain.
Keywords
Adeno-associated virus (rAAV), Brain, Gene delivery, Stereotaxic surgery
Publication Date
1-1-2016
Publication Title
Methods in Molecular Biology
ISSN
10643745
Volume
1382
First Page
199
Last Page
215
PubMed ID
26611588
Digital Object Identifier (DOI)
10.1007/978-1-4939-3271-9_14
Recommended Citation
Benskey, Matthew J. and Manfredsson, Fredric P., "Intraparenchymal stereotaxic delivery of rAAV and special considerations in vector handling" (2016). Translational Neuroscience. 1429.
https://scholar.barrowneuro.org/neurobiology/1429