A draft map of the human proteome.
Document Type
Article
Abstract
The availability of human genome sequence has transformed biomedical research over the past decade. However, an equivalent map for the human proteome with direct measurements of proteins and peptides does not exist yet. Here we present a draft map of the human proteome using high-resolution Fourier-transform mass spectrometry. In-depth proteomic profiling of 30 histologically normal human samples, including 17 adult tissues, 7 fetal tissues and 6 purified primary haematopoietic cells, resulted in identification of proteins encoded by 17,294 genes accounting for approximately 84% of the total annotated protein-coding genes in humans. A unique and comprehensive strategy for proteogenomic analysis enabled us to discover a number of novel protein-coding regions, which includes translated pseudogenes, non-coding RNAs and upstream open reading frames. This large human proteome catalogue (available as an interactive web-based resource at http://www.humanproteomemap.org) will complement available human genome and transcriptome data to accelerate biomedical research in health and disease.
Keywords
Adult, Cells, Cultured, Databases, Protein, Fetus, Fourier Analysis, Gene Expression Profiling, Genome, Human, Hematopoietic Stem Cells, Humans, Internet, Mass Spectrometry, Molecular Sequence Annotation, Open Reading Frames, Organ Specificity, Protein Biosynthesis, Protein Isoforms, Protein Sorting Signals, Protein Transport, Proteome, Proteomics, Pseudogenes, RNA, Untranslated, Reproducibility of Results, Untranslated Regions
Medical Subject Headings
Adult; Cells, Cultured; Databases, Protein; Fetus; Fourier Analysis; Gene Expression Profiling; Genome, Human; Hematopoietic Stem Cells; Humans; Internet; Mass Spectrometry; Molecular Sequence Annotation; Open Reading Frames; Organ Specificity; Protein Biosynthesis; Protein Isoforms; Protein Sorting Signals; Protein Transport; Proteome; Proteomics; Pseudogenes; RNA, Untranslated; Reproducibility of Results; Untranslated Regions
Publication Date
5-29-2014
Publication Title
Nature
ISSN
1476-4687
Volume
509
Issue
7502
First Page
575
Last Page
581
PubMed ID
24870542
Digital Object Identifier (DOI)
10.1038/nature13302
Recommended Citation
Kim, Min-Sik; Pinto, Sneha M; Getnet, Derese; Nirujogi, Raja Sekhar; Manda, Srikanth S; Chaerkady, Raghothama; Madugundu, Anil K; Kelkar, Dhanashree S; Isserlin, Ruth; Jain, Shobhit; Thomas, Joji K; Muthusamy, Babylakshmi; Leal-Rojas, Pamela; Kumar, Praveen; Sahasrabuddhe, Nandini A; Balakrishnan, Lavanya; Advani, Jayshree; George, Bijesh; Renuse, Santosh; Selvan, Lakshmi Dhevi N; Patil, Arun H; Nanjappa, Vishalakshi; Radhakrishnan, Aneesha; Prasad, Samarjeet; Subbannayya, Tejaswini; Raju, Rajesh; Kumar, Manish; Sreenivasamurthy, Sreelakshmi K; Marimuthu, Arivusudar; Sathe, Gajanan J; Chavan, Sandip; Datta, Keshava K; Subbannayya, Yashwanth; Sahu, Apeksha; Yelamanchi, Soujanya D; Jayaram, Savita; Rajagopalan, Pavithra; Sharma, Jyoti; Murthy, Krishna R; Syed, Nazia; Goel, Renu; Khan, Aafaque A; Ahmad, Sartaj; Dey, Gourav; Mudgal, Keshav; Chatterjee, Aditi; Huang, Tai-Chung; Zhong, Jun; Wu, Xinyan; Shaw, Patrick G; Freed, Donald; Zahari, Muhammad S; Mukherjee, Kanchan K; Shankar, Subramanian; Mahadevan, Anita; Lam, Henry; Mitchell, Christopher J; Shankar, Susarla Krishna; Satishchandra, Parthasarathy; Schroeder, John T; Sirdeshmukh, Ravi; Maitra, Anirban; Leach, Steven D; Drake, Charles G; Halushka, Marc K; Prasad, T S Keshava; Hruban, Ralph H; Kerr, Candace L; Bader, Gary D; Iacobuzio-Donahue, Christine A; Gowda, Harsha; and Pandey, Akhilesh, "A draft map of the human proteome." (2014). Translational Neuroscience. 2447.
https://scholar.barrowneuro.org/neurobiology/2447