Long-Term Storage Effects on Stability of Aβ, Aβ, and Total Tau Proteins in Human Plasma Samples Measured with Immunomagnetic Reduction Assays
Background: The stability of Alzheimer's disease (AD) biomarkers in plasma, measured by immunomagnetic reduction (IMR) after long-term storage at -80°C, has not been established before. Method: Ninety-nine human plasma samples from 53 normal controls (NCs), 5 patients with amnestic mild cognitive impairment (aMCI), and 41 AD patients were collected. Each plasma sample was aliquoted and stored as single-use aliquots at -80°C. The baseline measurements for Aβ, Aβ, and total Tau protein (T-Tau) concentrations for each sample were done within 3 months of blood draw by IMR. They are referred to as baseline concentrations. A separate aliquot from each sample was assayed with IMR to assess the stability of the measured analytes during storage at -80°C between 1.1 and 5.4 years. This is referred to as a repeated result. Results: IMR shows that plasma levels of Aβ and Aβ exhibit stability over 5-year storage at -80°C and that plasma levels of T-Tau are less stable (approximately 1.5 years). Conclusion: Although the measured concentrations of T-Tau in human plasma may alter during storage, the diagnostic utility of the results are only slightly affected when the product of Aβ and T-Tau concentrations are used. The results show that the overall agreement between baseline and repeated measurements in the ability of discriminating NCs from aMCI/AD patients is higher than 80%.
Dementia and geriatric cognitive disorders extra
Digital Object Identifier (DOI)
Chiu, Ming-Jang; Lue, Lih-Fen; Sabbagh, Marwan N.; Chen, Ta-Fu; Chen, H H.; and Yang, Shieh-Yueh, "Long-Term Storage Effects on Stability of Aβ, Aβ, and Total Tau Proteins in Human Plasma Samples Measured with Immunomagnetic Reduction Assays" (2019). Neurology. 932.