Long-Term Storage Effects on Stability of Aβ, Aβ, and Total Tau Proteins in Human Plasma Samples Measured with Immunomagnetic Reduction Assays

Document Type

Article

Abstract

Background: The stability of Alzheimer's disease (AD) biomarkers in plasma, measured by immunomagnetic reduction (IMR) after long-term storage at -80°C, has not been established before. Method: Ninety-nine human plasma samples from 53 normal controls (NCs), 5 patients with amnestic mild cognitive impairment (aMCI), and 41 AD patients were collected. Each plasma sample was aliquoted and stored as single-use aliquots at -80°C. The baseline measurements for Aβ, Aβ, and total Tau protein (T-Tau) concentrations for each sample were done within 3 months of blood draw by IMR. They are referred to as baseline concentrations. A separate aliquot from each sample was assayed with IMR to assess the stability of the measured analytes during storage at -80°C between 1.1 and 5.4 years. This is referred to as a repeated result. Results: IMR shows that plasma levels of Aβ and Aβ exhibit stability over 5-year storage at -80°C and that plasma levels of T-Tau are less stable (approximately 1.5 years). Conclusion: Although the measured concentrations of T-Tau in human plasma may alter during storage, the diagnostic utility of the results are only slightly affected when the product of Aβ and T-Tau concentrations are used. The results show that the overall agreement between baseline and repeated measurements in the ability of discriminating NCs from aMCI/AD patients is higher than 80%.

Publication Date

1-1-2019

Publication Title

Dementia and geriatric cognitive disorders extra

ISSN

1664-5464

Volume

9

Issue

1

First Page

77

Last Page

86

PubMed ID

31043966

Digital Object Identifier (DOI)

10.1159/000496099

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